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While, ELISA IgG specificity in all groups was (80%) and the sensitivity in acute brucellosis was (88%), in subacute and chronic brucellosis was (100%). Results: Serum IgG and IgM for brucellosis by ELISA test had increased values in confirmed brucella cases, ELISA IgM was highly specific (100%) in all groups and sensitive (96%) in acute brucellosis, (100%) in subacute brucellosis and (64%) in chronic brucellosis. Culture positivity for brucellosis was used as the reference standard for diagnosis.
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Patients and Methods: Sera from 150 individuals from confirmed brucellosis cases and 25 healthy individuals were tested for presence of IgG and IgM antibodies by ELISA assay.
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The aim of this article is to study the pattern of the brucellosis in Menoufyia governorate. Abstract: Background and study aim : ELISA can determine specific antibody classes against brucella, It is a sensitive, simple and rapid test, thus help to study the pattern of the brucellosis.
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